diff --git a/common/thread_flags.aconfig b/common/thread_flags.aconfig
index 14b70d0563..60120bcc54 100644
--- a/common/thread_flags.aconfig
+++ b/common/thread_flags.aconfig
@@ -35,3 +35,12 @@ flag {
     description: "Controls whether the Android Thread Ephemeral Key feature is enabled"
     bug: "348323500"
 }
+
+flag {
+    name: "set_nat64_configuration_enabled"
+    is_exported: true
+    is_fixed_read_only: true
+    namespace: "thread_network"
+    description: "Controls whether the setConfiguration API of NAT64 feature is enabled"
+    bug: "368456504"
+}
diff --git a/framework-t/api/system-current.txt b/framework-t/api/system-current.txt
index 09a3681f4e..08129eb8e3 100644
--- a/framework-t/api/system-current.txt
+++ b/framework-t/api/system-current.txt
@@ -506,6 +506,13 @@ package android.net.thread {
     field @NonNull public static final android.os.Parcelable.Creator<android.net.thread.ThreadConfiguration> CREATOR;
   }
 
+  @FlaggedApi("com.android.net.thread.flags.set_nat64_configuration_enabled") public static final class ThreadConfiguration.Builder {
+    ctor @FlaggedApi("com.android.net.thread.flags.set_nat64_configuration_enabled") public ThreadConfiguration.Builder();
+    ctor @FlaggedApi("com.android.net.thread.flags.set_nat64_configuration_enabled") public ThreadConfiguration.Builder(@NonNull android.net.thread.ThreadConfiguration);
+    method @FlaggedApi("com.android.net.thread.flags.set_nat64_configuration_enabled") @NonNull public android.net.thread.ThreadConfiguration build();
+    method @FlaggedApi("com.android.net.thread.flags.set_nat64_configuration_enabled") @NonNull public android.net.thread.ThreadConfiguration.Builder setNat64Enabled(boolean);
+  }
+
   @FlaggedApi("com.android.net.thread.flags.thread_enabled") public final class ThreadNetworkController {
     method @FlaggedApi("com.android.net.thread.flags.epskc_enabled") @RequiresPermission("android.permission.THREAD_NETWORK_PRIVILEGED") public void activateEphemeralKeyMode(@NonNull java.time.Duration, @NonNull java.util.concurrent.Executor, @NonNull android.os.OutcomeReceiver<java.lang.Void,android.net.thread.ThreadNetworkException>);
     method public void createRandomizedDataset(@NonNull String, @NonNull java.util.concurrent.Executor, @NonNull android.os.OutcomeReceiver<android.net.thread.ActiveOperationalDataset,android.net.thread.ThreadNetworkException>);
@@ -520,6 +527,7 @@ package android.net.thread {
     method @RequiresPermission(android.Manifest.permission.ACCESS_NETWORK_STATE) public void registerStateCallback(@NonNull java.util.concurrent.Executor, @NonNull android.net.thread.ThreadNetworkController.StateCallback);
     method @RequiresPermission("android.permission.THREAD_NETWORK_PRIVILEGED") public void scheduleMigration(@NonNull android.net.thread.PendingOperationalDataset, @NonNull java.util.concurrent.Executor, @NonNull android.os.OutcomeReceiver<java.lang.Void,android.net.thread.ThreadNetworkException>);
     method @FlaggedApi("com.android.net.thread.flags.channel_max_powers_enabled") @RequiresPermission("android.permission.THREAD_NETWORK_PRIVILEGED") public void setChannelMaxPowers(@NonNull @Size(min=1) android.util.SparseIntArray, @NonNull java.util.concurrent.Executor, @NonNull android.os.OutcomeReceiver<java.lang.Void,android.net.thread.ThreadNetworkException>);
+    method @FlaggedApi("com.android.net.thread.flags.set_nat64_configuration_enabled") @RequiresPermission(android.Manifest.permission.THREAD_NETWORK_PRIVILEGED) public void setConfiguration(@NonNull android.net.thread.ThreadConfiguration, @NonNull java.util.concurrent.Executor, @NonNull android.os.OutcomeReceiver<java.lang.Void,android.net.thread.ThreadNetworkException>);
     method @RequiresPermission("android.permission.THREAD_NETWORK_PRIVILEGED") public void setEnabled(boolean, @NonNull java.util.concurrent.Executor, @NonNull android.os.OutcomeReceiver<java.lang.Void,android.net.thread.ThreadNetworkException>);
     method @FlaggedApi("com.android.net.thread.flags.configuration_enabled") @RequiresPermission(android.Manifest.permission.THREAD_NETWORK_PRIVILEGED) public void unregisterConfigurationCallback(@NonNull java.util.function.Consumer<android.net.thread.ThreadConfiguration>);
     method @RequiresPermission(allOf={android.Manifest.permission.ACCESS_NETWORK_STATE, "android.permission.THREAD_NETWORK_PRIVILEGED"}) public void unregisterOperationalDatasetCallback(@NonNull android.net.thread.ThreadNetworkController.OperationalDatasetCallback);
diff --git a/thread/framework/java/android/net/thread/ThreadConfiguration.java b/thread/framework/java/android/net/thread/ThreadConfiguration.java
index 1c2553511a..e6fa1efd1e 100644
--- a/thread/framework/java/android/net/thread/ThreadConfiguration.java
+++ b/thread/framework/java/android/net/thread/ThreadConfiguration.java
@@ -126,18 +126,29 @@ public final class ThreadConfiguration implements Parcelable {
      *
      * @hide
      */
+    @FlaggedApi(Flags.FLAG_SET_NAT64_CONFIGURATION_ENABLED)
+    @SystemApi
     public static final class Builder {
         private boolean mNat64Enabled = false;
         private boolean mDhcpv6PdEnabled = false;
 
-        /** Creates a new {@link Builder} object with all features disabled. */
+        /**
+         * Creates a new {@link Builder} object with all features disabled.
+         *
+         * @hide
+         */
+        @FlaggedApi(Flags.FLAG_SET_NAT64_CONFIGURATION_ENABLED)
+        @SystemApi
         public Builder() {}
 
         /**
          * Creates a new {@link Builder} object from a {@link ThreadConfiguration} object.
          *
          * @param config the Border Router configurations to be copied
+         * @hide
          */
+        @FlaggedApi(Flags.FLAG_SET_NAT64_CONFIGURATION_ENABLED)
+        @SystemApi
         public Builder(@NonNull ThreadConfiguration config) {
             Objects.requireNonNull(config);
 
@@ -150,7 +161,11 @@ public final class ThreadConfiguration implements Parcelable {
          *
          * <p>Enabling this feature will allow Thread devices to connect to the internet/cloud over
          * IPv4.
+         *
+         * @hide
          */
+        @FlaggedApi(Flags.FLAG_SET_NAT64_CONFIGURATION_ENABLED)
+        @SystemApi
         @NonNull
         public Builder setNat64Enabled(boolean enabled) {
             this.mNat64Enabled = enabled;
@@ -162,6 +177,8 @@ public final class ThreadConfiguration implements Parcelable {
          *
          * <p>Enabling this feature will allow Thread devices to connect to the internet/cloud over
          * IPv6.
+         *
+         * @hide
          */
         @NonNull
         public Builder setDhcpv6PdEnabled(boolean enabled) {
@@ -169,7 +186,13 @@ public final class ThreadConfiguration implements Parcelable {
             return this;
         }
 
-        /** Creates a new {@link ThreadConfiguration} object. */
+        /**
+         * Creates a new {@link ThreadConfiguration} object.
+         *
+         * @hide
+         */
+        @FlaggedApi(Flags.FLAG_SET_NAT64_CONFIGURATION_ENABLED)
+        @SystemApi
         @NonNull
         public ThreadConfiguration build() {
             return new ThreadConfiguration(this);
diff --git a/thread/framework/java/android/net/thread/ThreadNetworkController.java b/thread/framework/java/android/net/thread/ThreadNetworkController.java
index 1222398f67..bac2501c5a 100644
--- a/thread/framework/java/android/net/thread/ThreadNetworkController.java
+++ b/thread/framework/java/android/net/thread/ThreadNetworkController.java
@@ -748,15 +748,19 @@ public final class ThreadNetworkController {
      * OutcomeReceiver#onResult} will be called, and the {@code configuration} will be applied and
      * persisted to the device; the configuration changes can be observed by {@link
      * #registerConfigurationCallback}. On failure, {@link OutcomeReceiver#onError} of {@code
-     * receiver} will be invoked with a specific error.
+     * receiver} will be invoked with a specific error:
+     *
+     * <ul>
+     *   <li>{@link ThreadNetworkException#ERROR_UNSUPPORTED_FEATURE} the configuration enables a
+     *       feature which is not supported by the platform.
+     * </ul>
      *
      * @param configuration the configuration to set
      * @param executor the executor to execute {@code receiver}
      * @param receiver the receiver to receive result of this operation
-     * @hide
      */
-    // @FlaggedApi(ThreadNetworkFlags.FLAG_CONFIGURATION_ENABLED)
-    // @RequiresPermission(permission.THREAD_NETWORK_PRIVILEGED)
+    @FlaggedApi(Flags.FLAG_SET_NAT64_CONFIGURATION_ENABLED)
+    @RequiresPermission(permission.THREAD_NETWORK_PRIVILEGED)
     public void setConfiguration(
             @NonNull ThreadConfiguration configuration,
             @NonNull @CallbackExecutor Executor executor,
diff --git a/thread/tests/cts/src/android/net/thread/cts/ThreadNetworkControllerTest.java b/thread/tests/cts/src/android/net/thread/cts/ThreadNetworkControllerTest.java
index 1792bfb37a..07438e9a9c 100644
--- a/thread/tests/cts/src/android/net/thread/cts/ThreadNetworkControllerTest.java
+++ b/thread/tests/cts/src/android/net/thread/cts/ThreadNetworkControllerTest.java
@@ -182,6 +182,7 @@ public class ThreadNetworkControllerTest {
     @After
     public void tearDown() throws Exception {
         dropAllPermissions();
+        setEnabledAndWait(mController, true);
         leaveAndWait(mController);
         tearDownTestNetwork();
         setConfigurationAndWait(mController, DEFAULT_CONFIG);
@@ -1148,15 +1149,9 @@ public class ThreadNetworkControllerTest {
         CompletableFuture<Void> setFuture2 = new CompletableFuture<>();
         ConfigurationListener listener = new ConfigurationListener(mController);
         ThreadConfiguration config1 =
-                new ThreadConfiguration.Builder()
-                        .setNat64Enabled(true)
-                        .setDhcpv6PdEnabled(true)
-                        .build();
+                new ThreadConfiguration.Builder().setNat64Enabled(true).build();
         ThreadConfiguration config2 =
-                new ThreadConfiguration.Builder()
-                        .setNat64Enabled(false)
-                        .setDhcpv6PdEnabled(true)
-                        .build();
+                new ThreadConfiguration.Builder().setNat64Enabled(false).build();
 
         try {
             runAsShell(